Journal: Immunology

Article Title: Frequency and phenotype of natural killer cells and natural killer cell subsets in bovine lymphoid compartments and blood

doi: 10.1111/imm.12708

Figure Lengend Snippet: CD2 − natural killer (NK) cells are the predominant subset of NK cells present within skin‐draining afferent lymph (AL). Lymphocytes derived from peripheral blood (PB), AL and the lymph nodes (LNs) of seven calves were labelled with monoclonal antibodies to NKp46, CD3 and CD2 and analysed by flow cytometry. FACS plots from one representative animal illustrate the expression of NKp46 and CD3 by PB‐, AL‐ and LN‐derived lymphocytes (a). FACS plots from one representative animal illustrate the expression of NKp46 and CD2 by PB‐, AL‐ and LN‐derived lymphocytes (b). Gates were set based on Fluorescence Minus One controls. Pooled data from seven calves indicate the average percentage of NKp46 + CD3 − NK cells ± SD present in PB (circles), AL (squares) and LNs (triangles) (c). Pooled data from seven calves illustrate the average percentage of CD2 + (lighter bars) and CD2 − (darker bars) NK cells ± SD within the total gated NKp46 + NK cell population from PB, AL or the LNs (d). Data were normally distributed ( P > 0·05). Significance between PB and AL was assessed using a paired t ‐test and significance between PB and LN was assessed using a two‐sample t ‐test. P < 0·001***. [Colour figure can be viewed at wileyonlinelibrary.com ]

Article Snippet: NK cells were identified by labelling cells with mouse anti‐bovine CD3 (MM1A, IgG1, VMRD, WA) indirectly conjugated to goat anti‐mouse IgG1‐AF647 (Life Technologies, Warrington, UK), phycoerythrin‐conjugated mouse anti‐bovine CD335 (AKS1, IgG1; Bio‐Rad AbD Serotec, Kidlington, UK) or mouse anti‐bovine CD335 (AKS6, IgG2b; a gift from Professor A.K.

Techniques: Derivative Assay, Flow Cytometry, Expressing, Fluorescence

Journal: Immunology

Article Title: Frequency and phenotype of natural killer cells and natural killer cell subsets in bovine lymphoid compartments and blood

doi: 10.1111/imm.12708

Figure Lengend Snippet: Natural killer (NK) cells are present in bovine efferent lymph and CD2 − NK cells are the principal subset present. Lymphocytes derived from peripheral blood (PB), afferent lymph (AL), lymph nodes (LNs) and efferent lymph (EL) were labelled with monoclonal antibodies for NKp46, CD3 and CD2 and analysed by flow cytometry. Pooled data from seven calves for PB, AL and LNs and five calves for EL, show the average percentage of NKp46 + CD3 − NK cells ± SD present within PB (circles), AL (squares), LNs (triangles) and EL (diamonds) (a). Pooled data from seven (PB, AL and LNs) and five (EL) calves illustrate the average percentage of NKp46 + CD2 + (lighter bars) and NKp46 + CD2 − (darker bars) NK cells ± SD within the total gated NKp46 + NK cell population from PB, AL, LNs and five calves for EL (b). Data were normally distributed ( P > 0·05) and significance between PB and EL was assessed using a two‐sample t ‐test. P < 0·01**, P < 0·001***.

Article Snippet: NK cells were identified by labelling cells with mouse anti‐bovine CD3 (MM1A, IgG1, VMRD, WA) indirectly conjugated to goat anti‐mouse IgG1‐AF647 (Life Technologies, Warrington, UK), phycoerythrin‐conjugated mouse anti‐bovine CD335 (AKS1, IgG1; Bio‐Rad AbD Serotec, Kidlington, UK) or mouse anti‐bovine CD335 (AKS6, IgG2b; a gift from Professor A.K.

Techniques: Derivative Assay, Flow Cytometry

Journal: Journal of Translational Medicine

Article Title: Endothelial glycocalyx shedding and vascular permeability in severely injured trauma patients

doi: 10.1186/s12967-015-0481-5

Figure Lengend Snippet: Summary of measured glycocalyx components, catecholamines and ECIS resistance

Article Snippet: Commercial enzyme-linked immunosorbant assays (ELISAs) for soluble syndecan-1, chondroitin sulfate, heparan sulfate and hyaluronic acid were performed to quantify levels of endothelial glycocalyx component degradations (Syndecan-1: Abcam, Cat. No. ab46506, Cambridge, MA; Heparan Sulfate: Biotang, Cat. No. HU8718, Lexington, MA; Chondroitin Sulfate: Biotang, Cat. No. HU8720, Lexington, MA; Hyaluronic Acid: R&D Systems, Cat. No. DHYAL0, Minneapolis, MN).

Techniques:

Journal: Frontiers in Veterinary Science

Article Title: One Health in Action: Operational Aspects of an Integrated Surveillance System for Zoonoses in Western Kenya

doi: 10.3389/fvets.2019.00252

Figure Lengend Snippet: Biological samples collected, laboratory tests performed, and pathogens investigated, during an integrated surveillance program for zoonoses in western Kenya.

Article Snippet: , , Toxoplasma spp . (Anti-Toxoplasma gondii IgG Human ELISA Kit [Abcam] and Toxoplasma IgM ELISA [DRG International]) , As per manufacturer's instructions.

Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Stripping Membranes, Microscopy